FastQCFastQC Report
Wed 25 May 2016
SRR1781063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262400
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6870926.18483231707317No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG85233.248094512195122No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC41831.5941310975609757No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT24870.9477896341463414No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC17940.6836890243902439No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT17730.6756859756097561RNA PCR Primer, Index 19 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC12470.47522865853658536No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG6310.24047256097560976No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC6170.23513719512195122RNA PCR Primer, Index 19 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG5210.19855182926829268No Hit
CTTATACACATCTCCGAGCCAACGAGACTTATGAAAATCTCGTATGCCGT5090.19397865853658536No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC4590.17492378048780488No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC4550.17339939024390244No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG4400.1676829268292683No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT3660.13948170731707316No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT3650.13910060975609756RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC3510.13376524390243905No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT3380.1288109756097561No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3340.12728658536585366No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT3330.12690548780487806No Hit
CTTATACACATCTCCGAGACCACGAGACTTATGAAAATCTCGTATGCCGT3190.12157012195121951No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT3030.11547256097560975No Hit
CTTATACACATCTCAGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2850.1086128048780488No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG2650.10099085365853659No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA207.905389E-570.02116411
ATAACCG207.905389E-570.02116410
AACCGAG207.912858E-570.0078212
TCCACGA150.002222002370.0078112
AGCCGAC301.0217627E-769.9944845
TGGACAC150.002223682669.9944825
TGACGAA150.002223682669.9944845
CCGTCAA207.9203324E-569.9944847
TCGTATT150.002223682669.9944840
GTTCAGC150.002223682669.9944821
AACTCGA150.002223682669.9944837
CGTCATA150.002223682669.9944848
GCCGTAA252.836212E-669.9944846
AACACGT150.002223682669.9944837
AGGGGGG82050.064.49216570
CGTCTAC1000.062.9950348
ACTCCGA1250.061.6186211
ACGTATG750.060.66187740
GCCGACT1100.060.44977646
TAGACTT352.9686817E-759.99526624
Produced by FastQC (version 0.11.5)