FastQCFastQC Report
Wed 25 May 2016
SRR1781062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347021
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7217120.79730045155769No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA150.002223628369.9997166
TATGCGC150.002223628369.999713
GAGTACC200.006948080452.49978327
TTACCGT200.006948080452.49978332
GCGGGTC352.0844866E-549.9997946
CGCGTGT305.873819E-446.6664737
TGCGCGT404.588256E-543.749825
TGGCGGC350.001254583139.99983619
ATTCGCC350.001254583139.99983645
CGACAGG350.001254583139.99983625
TGCGGGT350.001254583139.9998365
GACTTTC350.001254583139.99983663
TCGGTGG901.05501385E-1038.8887345
GCGCGTC556.685892E-638.181666
TCGCCGT400.002417162834.99985552
CCGATCC400.002417162834.99985563
AGCCCGA501.70807E-434.99985526
GTCTCGG601.21413905E-534.99985542
CACGCTA400.002417162834.99985523
CATTAGT400.002417162834.99985542
Produced by FastQC (version 0.11.5)