FastQCFastQC Report
Wed 25 May 2016
SRR1781062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347021
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4627113.333775189397759No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG74762.154336481077514No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC29820.859313989643278No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT14490.41755398088300133Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC13630.3927716190086479No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC9930.28614982954922036No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT8360.2409076107785984No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG4360.1256408113630011No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4050.1167076344082923No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT3840.11065612743897343No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC3780.10892712544773948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG57500.062.6956670
CGTCTTA1000.059.49999648
AATCTCG1350.057.03703736
CGAATGC1050.056.6666741
CGTATGA1500.056.041
TATGACG1650.055.15151243
TGACGTC1500.053.66666845
CACGAAT200.00694796852.539
CGAATAT200.00694796852.530
TTTTCGA200.00694796852.526
CTTTATA2200.050.909092
CGTCTTC76100.050.36136648
CCGTCTT75850.050.3427847
GCCGTCT76300.050.22935546
TCTTCTG77000.050.09090850
CTGCTTG74650.050.07367754
TCTCGTA78300.050.01915438
TACGAGA352.084436E-550.013
TCGTATG78900.049.9936640
CTTCTGC76450.049.99345851
Produced by FastQC (version 0.11.5)