FastQCFastQC Report
Wed 25 May 2016
SRR1781061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92007
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7059976.73220515830317No Hit
CTTATACACATCTGACGCTGCCGACGACAAGTTAAGTGTAGATCTCGGTG1920.208679774364994No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1160.1260773636788505No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950.10325301335767931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGG150.002218386270.000015
TGCCGGC150.002218386270.0000119
CGACGAT150.002218386270.0000122
CGTCGGG150.002218386270.000019
TCGGGCG150.002218386270.0000111
GCCGGCG252.3794532E-456.00000420
ATGTTTA200.00693182852.535
CTGCCGG200.00693182852.518
CGGCTGG200.00693182852.59
GTGCGCG200.00693182852.54
GGTGTAG200.00693182852.535
TTATGTT200.00693182852.533
CGTATCA604.8567017E-946.66666856
TGGCGCT305.8510894E-446.66666813
CCGGCGA305.8510894E-446.66666821
TATCATT551.2950659E-744.54545258
GTATCAT659.853466E-943.07692357
CCGTATC602.5651752E-740.83333655
TCTCGGT602.5651752E-740.83333643
GCCGTAT602.5651752E-740.83333654
Produced by FastQC (version 0.11.5)