FastQCFastQC Report
Wed 25 May 2016
SRR1781061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92007
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4571349.68426315389046No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG71197.737454758877043No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC23172.5182866521025575No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC13071.4205440890367036No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT11161.2129511884965274RNA PCR Primer, Index 34 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC8800.9564489658395557No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT7470.8118947471388046No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG4000.4347495299270708No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT3550.3858402078102753No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT3520.3825795863358223No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC3510.3814927125110046No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT3280.35649461454019804No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3200.3477996239416566No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3160.3434521286423859No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC2980.3238883997956677RNA PCR Primer, Index 34 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACCTCATGGGATCTCGTATGCCGT2780.30215092329931414No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT2770.3010640494744965No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT2600.282587194452596No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT2590.2815003206277783RNA PCR Primer, Index 34 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2570.279326572978143No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGATCTCGTATGCCGTC2570.279326572978143No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2200.23911224145988894No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT2110.22933037703652984No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCAGT1990.21628789113871771No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT1890.20541915289054094No Hit
CTTATACACATCTCAGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1580.17172606432119294No Hit
CTTATACACATCTCCGAGACCACGAGACCTCATGGGATCTCGTATGCCGT1510.16411794754746922No Hit
TCTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1510.16411794754746922No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1440.15650983077374547No Hit
CTTATAAACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1250.1358592281022096No Hit
CTTATACAAATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT970.10542676100731466No Hit
CTCTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG950.10325301335767931No Hit
CTTATACACATCTACGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT950.10325301335767931No Hit
CTTATACACATCTCCGAGCACACGAGACCTCATGGGATCTCGTATGCCGT940.10216613953286163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAG150.002218386270.0000154
ACTAATG150.002218386270.0000128
AAGTAAG150.002218386270.0000140
AATCTAC150.002218386270.000019
AAATCTA150.002218386270.000018
TCCTATG150.002218386270.0000140
ATGACGA150.002218386270.0000144
TAAGCAG150.002218386270.0000143
CAACTCA150.002218386270.000019
TATGCCC150.002218386270.0000143
ACGTATT150.002218386270.0000147
ACGTATG150.002218386270.0000140
ACGTAAT252.8202576E-670.0000147
ACTCCGA150.002218386270.0000111
CTGATAG150.002218386270.0000154
CATCAAG150.002218386270.0000128
TCAGCTA150.002218386270.0000153
AACTCCG150.002218386270.0000110
ACTCAGA150.002218386270.0000111
TTGGGAT150.002218386270.0000133
Produced by FastQC (version 0.11.5)