FastQCFastQC Report
Wed 25 May 2016
SRR1781060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249662
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8082932.37537150227107No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG150.002221194670.013222
GCGCGTC252.834673E-669.99926
CCGCTCT150.0022229669.999264
TACGCGT150.0022229669.99925
CGCGTCT301.0210897E-769.99927
ATCGATA207.916933E-569.999223
CCCGATC207.916933E-569.999233
TGCGCGT352.9667171E-759.9993135
AATCGAT252.3875358E-455.9993622
GGCGCGT200.006946010552.49945
CGCCGTG200.006946010552.499453
GTGCCGC200.006946010552.499461
TGGTCGC506.175833E-848.9994449
TCGCCGT451.680588E-646.66613452
GCGCGTG305.870846E-446.6661346
TGTGCGC305.870846E-446.6661343
CGCGTGT305.870846E-446.6661347
TGCCGGC604.9149094E-946.66613419
CTCGGTG751.4551915E-1146.6661344
GGCGCTG851.8189894E-1245.293614
Produced by FastQC (version 0.11.5)