FastQCFastQC Report
Wed 25 May 2016
SRR1781060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249662
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT5208820.86340732670571No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG80133.2095392971297194No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC27131.0866691767269347No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC14740.590398218391265No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT13070.5235077825219697TruSeq Adapter, Index 10 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT10460.41896644263043636No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC9560.3829177047368042No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4760.19065776930409914No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG4660.18665235398258445No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT3910.1566117390712243No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3720.1490014499603464No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3610.14459549310668024No Hit
CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT3520.14099061931731702No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC3470.13898791165655966No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT3360.13458195480289353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3180.12737220722416706No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC3160.12657112415986413No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT2860.11455487819532006No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG2670.10694458908444217No Hit
CTTATACACATCTCCGAGACCACGAGACACCGGCTAATCTCGTATGCCGT2550.10213809069862453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT150.00222316269.997646
ATCGTAA207.917831E-569.997639
TAACCGA207.917831E-569.997611
ATGACGA252.8350569E-669.997644
GCCGTAA252.8350569E-669.997646
AGGGGGG63550.064.7656870
TGCCGTA453.8380676E-1062.22008545
CACCGGA650.059.22873328
GGCTAAA1250.058.7979832
CATCTAA308.362673E-658.331339
ATATCGA308.362673E-658.3313337
CGTAATC308.362673E-658.3313348
CCGGCAA1450.057.92904730
AAACTCG1100.057.2707636
ACCGGAT553.6379788E-1157.2707629
TCTACGA800.056.8730511
CAGCTTG1000.055.99807754
ATAACCG252.3878057E-455.99807710
GCCGTAT252.3878057E-455.99807746
ACCGGCA1450.055.51533529
Produced by FastQC (version 0.11.5)