FastQCFastQC Report
Wed 25 May 2016
SRR1781059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229691
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6939230.21102263475713No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATG207.915257E-569.9997913
GTCCGAT207.915257E-569.9997912
ACGTCCG150.00222265969.9997943
GACTCGC150.00222265969.9997931
CGCGTCT352.9654802E-759.9998137
CGATGTG308.3589985E-658.33315315
CGTCCGA252.3870335E-455.9998211
TGGTCGC509.767973E-1055.9998249
GAAATTC200.006945075552.499847
CTATCGG200.006945075552.4998442
TTGCATC200.006945075552.4998454
CGTCCGG200.006945075552.4998444
TCGCGAG200.006945075552.4998430
GTATCAT352.0822501E-549.99984457
TCGCCGT305.8696157E-446.66652352
CGTATCA305.8696157E-446.66652356
GCACGGT305.8696157E-446.66652348
CCTCGTC305.8696157E-446.6665238
CGACAAC305.8696157E-446.66652324
TCGTCCG305.8696157E-446.66652310
Produced by FastQC (version 0.11.5)