FastQCFastQC Report
Wed 25 May 2016
SRR1781059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229691
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4383919.08607651148717No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG66532.8965000805429906No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC25651.1167176772272314No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC12910.5620594624952654No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT10410.4532175836232155RNA PCR Primer, Index 14 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT7340.31955975636833833No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC7130.31041703854308617No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG3410.14846032278147597No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3340.14541275017305857No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3180.13844686992524738No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT3120.1358346648323182No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT3080.1340931947703654No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3000.1306102546464598No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2930.1275626820380424No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT2850.12407974191413682No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT2760.12016143427474302No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.11798459669730202No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT2610.11363092154242002No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT2610.11363092154242002No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT2330.10144063110875046No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCG207.884078E-570.0555655
CCTTACG150.002217561770.0403061
GACGTAT252.8350642E-669.9945646
TGACGTA252.8350642E-669.9945645
CGGATGC150.00222331769.994569
AACACGT150.00222331769.9945637
ACAGGTA207.918186E-569.9945527
AGGGGGG50950.063.67512570
TAAGACG352.9670082E-759.9953443
CGAATGC1050.059.9953341
ACCGGTA601.8189894E-1258.328827
ACGTATT308.362698E-658.328847
CGTCTTA900.058.32879348
CCGGTAC553.6379788E-1157.26827228
TCTACGA800.056.87057511
TCTTTAT2250.056.032241
AACTCGA252.3879137E-455.99564737
CGTCTAC700.054.99572448
ATCGTAT1050.053.3291839
CTTTATA2300.053.3031542
Produced by FastQC (version 0.11.5)