FastQCFastQC Report
Wed 25 May 2016
SRR1781058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293638
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7246624.678686001130643No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG150.002223271969.99982544
CCGGCGA350.001254257339.99990521
TCGGAGA350.001254257339.99990555
GTCGTGT400.002416539534.99991212
AGACTGC552.98811E-431.8181066
TCGTGTA552.98811E-431.81810613
CGTGTAG450.004303339431.11103614
CATCGCA450.004303339431.11103626
AGCCGAC500.007201851327.99993166
TCGTTCG500.007201851327.99993123
CCGGCGT500.007201851327.99993123
AACTTGG500.007201851327.9999318
CTTGGAT500.007201851327.9999313
ATGGTCA500.007201851327.99993123
TTCGGTA500.007201851327.99993126
CGCACTA500.007201851327.99993129
GATAGAT500.007201851327.99993111
TACACAG906.155178E-627.2221585
TCGGTAA657.945264E-426.92301227
TGGCGCT808.649257E-526.24993513
Produced by FastQC (version 0.11.5)