FastQCFastQC Report
Wed 25 May 2016
SRR1781058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293638
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT175285.9692546605003445No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT49681.691879116463128No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG17230.5867769157942773No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT14000.4767775287939572No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT8860.3017320646510329No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT8590.29253706945286373No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG8140.2772120774559151No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG6820.23225876759819913No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT6530.22238266164461No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC6210.211484889557891No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT5220.17776990716460406No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT5170.17606713027605417No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT4520.1539310307249062No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT4350.1481415893038367No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC3950.1345193741954379No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT3790.12907048815207842No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT3710.12634604513039865No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGG3510.11953493757619925No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT3190.10863716548948024No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCG3190.10863716548948024No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT150.002216391770.054541
TCGTTAG150.002223893869.99489640
TCTTACG150.002223893869.99489616
CGTTAGC150.002223893869.99489641
CCGACTT252.836954E-669.99489647
ATACGTA150.002223893869.99489645
ATCGCGA150.002223893869.99489637
AGGGGGG38800.064.49271470
TAATCCG501.4551915E-1162.99540343
TACGCGG453.8380676E-1062.21767429
AATCCGT401.0641088E-861.2455344
TGCGTCT401.0641088E-861.2455346
CCGAATT352.9697912E-759.99561747
AAGCCGT1350.059.62527544
CACGTAT650.059.22644839
TCGGAAG308.368244E-658.3290840
CGACTTA308.368244E-658.3290848
GCCGTGA308.368244E-658.3290846
AGCCGTC1150.057.82186545
CCGTCTT21200.057.77880547
Produced by FastQC (version 0.11.5)