FastQCFastQC Report
Wed 25 May 2016
SRR1781057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203965
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6477931.759860760424587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG150.002222335769.9995142
TGCCGGC252.3864437E-455.99960719
CGGGTGA200.006944074852.4996341
TCGGGCT200.006944074852.4996347
TTATCGC200.006944074852.4996310
TGTAGCG200.006944074852.4996316
CGGTCGT200.006944074852.4996310
TAGGACC200.006944074852.499634
TATCGCA200.006944074852.4996311
TACGTTC200.006944074852.4996320
CGTAAAC305.8681745E-446.666343
GCGCTGC451.6790109E-646.6663415
AGGTCGC404.581649E-543.7496940
GGCGCTG855.638867E-1141.1761814
GACACAT350.001253385239.99971826
GTGATCA350.001253385239.99971843
CGGTGGT701.915032E-839.99971846
AGAGCCA350.001253385239.99971849
TCGGTGG708.653824E-734.99975645
GATCGGA501.705635E-434.99975211
Produced by FastQC (version 0.11.5)