FastQCFastQC Report
Wed 25 May 2016
SRR1781057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203965
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT4074419.975976270438554No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG60112.9470742529355527No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC22831.1193096854852547No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT13570.6653102247934695No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT11420.5598999828401932Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC10880.5334248523030912No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT8930.43782021425244527No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC7410.36329762459245457No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG3810.18679675434510823No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC3510.1720883484911627No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3220.1578702228323487No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC3180.1559091020518226No Hit
CTTATACACATCTCCGAGCCAACGAGACGACGCTCCATCTCGTATGCCGT3010.1475743387345868No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2810.13776873483195645No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT2660.1304145319049837No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT2390.11717696663643272Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT2350.11521584585590665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2270.1112936042948545No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG2090.10246856078248719No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAT150.002223200269.99264570
AACGTAT150.002223200269.99264539
GACGTAT150.002223200269.99264546
TGACGTA207.9171325E-569.99264545
TACGAGA207.9171325E-569.99264513
CGAGACA207.9171325E-569.99264515
ACGTATT150.002223200269.99264547
ATACGAG150.002223200269.99264512
AGGGGGG50950.064.5663270
ACGTATG550.063.6296840
CCGACTT501.4551915E-1162.9933847
CGAAGCT501.4551915E-1162.9933828
ACGAAGC501.4551915E-1162.9933827
CACGTAT401.0622898E-861.2435739
CGACGCA601.8189894E-1258.32720628
TAGACGA308.360708E-658.32720624
CGTCTAC601.8189894E-1258.32720648
GATAGAC308.360708E-658.32720613
CGACTTC553.6379788E-1157.26671248
GACGAAG553.6379788E-1157.26671226
Produced by FastQC (version 0.11.5)