FastQCFastQC Report
Wed 25 May 2016
SRR1781056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348319
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7161320.559601974052523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG200.006948103752.49978351
GGTCGAC200.006948103752.49978350
TAGACGA200.006948103752.49978340
TTCGGTC200.006948103752.49978328
TGGTCGA200.006948103752.49978349
CGCGTCT506.184928E-848.9997987
TGTAACG305.8696925E-446.6731762
GCGCGTC451.6824324E-646.6664736
TTGCTCG305.8738486E-446.66647323
GGCGCGT305.8738486E-446.6664735
CCGGCGA350.001254589639.99983621
GTCTACT501.706641E-435.0048831
CGTGTTA400.002417175834.99985515
GTAACCC400.002417175834.99985549
TTGGGCG450.004301442331.1154522
TACATCG450.00430446631.11098320
TTCGGAA450.00430446631.11098367
AGCACCG703.4840326E-529.99987618
CGGTGGT952.815159E-729.47356246
CGTAGTG604.977367E-429.16654654
Produced by FastQC (version 0.11.5)