FastQCFastQC Report
Wed 25 May 2016
SRR1781056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348319
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4730513.580941608123586No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG71632.0564482557655483No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC28640.8222347905224808No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT12330.3539858577912775TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC12330.3539858577912775No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT9940.28537059419669897No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC8760.25149360212908284No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4420.12689517367700298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC750.065.3301248
AGGGGGG58150.064.03812470
AACTCGT601.8189894E-1258.33046337
GCCGTAT308.370325E-658.33046346
CCGACTT252.389297E-455.99724647
CGCGTTA252.389297E-455.99724628
ACGCGTT252.389297E-455.99724627
TATGACG1500.055.99724243
CGTATGA1650.055.14880441
GACGACT200.006949343752.49741446
CGAAGTA200.006949343752.49741428
ACGAAGT200.006949343752.49741427
CGTCTTC75450.052.4626348
TCGTATG78300.052.3856740
CCGTCTT74650.052.36848447
CTCGTAT77950.052.35149439
GCCGTCT75500.052.3351746
TATGCCG76400.052.31417543
TCTCGTA77900.052.25031738
AGATCTC76900.052.24710535