FastQCFastQC Report
Wed 25 May 2016
SRR1781055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470429
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7689916.34656877020762No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA200.00694978952.4994434
CGTATCA751.4551915E-1146.6661756
GTTGTGC404.576549E-543.77281
TCGCCGT802.910383E-1143.74953552
CTACCCG503.4767436E-641.9995510
CGGTGGT1100.041.363246
TCGGTGG1300.040.38418645
TCTTACG400.002418111334.99962628
CCAAACG400.002418111334.99962666
GCCGTAT957.625204E-933.15754354
TACCCGC652.1015472E-532.3073511
GCCGAGT652.1015472E-532.3073558
TATACGC552.9909727E-431.8178463
CTCGGTG1357.2759576E-1231.1107844
GTCGCCG1251.05501385E-1030.79967351
CAGTAGT604.964703E-429.1818661
CTGGCGC1352.582965E-1028.51821712
TGGTCGC1352.582965E-1028.51821749
TGCCGAG755.5815613E-527.99970257
TTCGTAC500.00720648327.999726
Produced by FastQC (version 0.11.5)