FastQCFastQC Report
Wed 25 May 2016
SRR1781055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470429
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT461509.810194524572251No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG67881.4429382542317757No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC28820.6126322994543278No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT19090.40579981251155856No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC12670.26932863407655566No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT8580.18238671510472357TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC7140.15177635732490982No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT6820.14497405559606233No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5350.11372598202916911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT501.4551915E-1162.99741446
AGGGGGG56550.061.88958770
GCAGCGT509.786163E-1056.0155641
CGTCTTC60200.053.1373648
GTCTTCT57550.053.02997249
GCCGTCT66950.052.85070846
CCGTCTT66850.052.82505447
CCGGATT200.006950624352.4978547
CGTCTAC200.006950624352.4978548
ACGTATT608.367351E-1152.4978547
CACGTAT200.006950624352.4978539
TATGCCG74350.052.39193343
TCTTCTG58500.052.28845250
TGCCGTC70300.052.27381545
TCTCGTA74400.052.0744838
CGTATGC71750.052.04664641
CTCGTAT72800.052.017139
ATGCCGT74700.052.005944
CTGCTTG58900.051.99277554
TTCTGCT57200.051.9471752