FastQCFastQC Report
Wed 25 May 2016
SRR1781054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209016
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7332435.080567994794656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT150.002222441869.9992821
CGAAAGG150.002222441869.999286
CATGCGA150.002222441869.9992857
GCGAACT150.002222441869.9992828
ACATTCG150.002222441869.9992830
ACGTCTG150.002222441869.999288
ATCGCCT150.002222441869.9992832
AGTCGTA150.002222441869.9992820
AAGTCGT150.002222441869.9992819
TGCGCGT308.356734E-658.3327335
CGCGTCT308.356734E-658.3327337
GATCTAA200.006931268652.5245931
ACGCGAT200.00694440352.49946259
ACACGTG200.00694440352.4994626
CAAGTCG200.00694440352.49946218
ACGTTAT200.00694440352.4994629
CCGGAAT200.00694440352.4994623
CGCGATA200.00694440352.49946260
TGCGGGT200.00694440352.4994625
TGCGGAA200.00694440352.49946219
Produced by FastQC (version 0.11.5)