FastQCFastQC Report
Wed 25 May 2016
SRR1781054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209016
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4820123.06091399701458No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG72483.467677115627512No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC28451.3611398170474986No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC13020.6229188196118957No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT11380.5444559268189996No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT10620.5080950740613159RNA PCR Primer, Index 19 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC7300.3492555593830137RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC4180.19998469016725995No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3850.18419642515405518No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT3790.1813258315152907RNA PCR Primer, Index 19 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG3770.18036896696903584No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT3470.1660159987752134No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3270.15644735331266504No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3250.15549048876641022No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3100.14831400466949898RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGACCACGAGACGCGTTAAAATCTCGTATGCCGT2780.13300417192942168RNA PCR Primer, Index 19 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC2690.1286982814712749No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT2670.1277414169250201No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG2600.12439239101312818No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCAGT2460.11769433918934437No Hit
CTTATACACATCTCAGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2450.11721590691621693RNA PCR Primer, Index 19 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT2390.11434531327745244No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT2150.10286293872239446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA150.002222923669.9954638
GTCTGAA150.002222923669.9954631
GGTCCAC150.002222923669.9954622
CACGAAT150.002222923669.9954639
TATTGCG150.002222923669.9954611
TCCAGTC150.002222923669.9954615
CTCGGTG150.002222923669.9954665
ACGTAAT150.002222923669.9954647
CGGTCCA150.002222923669.9954621
GTCCACG150.002222923669.9954623
CGCCGTT150.002222923669.9954670
TAGTGAA150.002222923669.995469
CTCTCGA150.002222923669.9954637
TATCGCG150.002222923669.9954649
GCCGTAA150.002222923669.9954646
ACGACTA150.002222923669.9954647
TAGACGC207.9160185E-569.9954524
ATCGTAA207.9160185E-569.9954539
ACGCGAA500.069.99544527
CGTAATC252.8339564E-669.99544548
Produced by FastQC (version 0.11.5)