FastQCFastQC Report
Wed 25 May 2016
SRR1781053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369068
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7844821.25570355598426No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT252.3892001E-455.9985932
GCCGTAT700.054.99861554
GAGTTCG200.006949006652.49868417
TGTACGT200.006949006652.49868412
GTACGTA200.006949006652.49868413
TCGTTGT352.0850066E-549.9987434
ATACACG305.874916E-446.6654934
CGTATCA851.8189894E-1245.29297656
TCGGTGG1100.044.54433445
CGCGTCT404.5893954E-543.74897
TCGCCGT901.8189894E-1242.776752
CTCGGTG901.8189894E-1242.776744
TGGTCGC1000.041.99894349
GTAGATC1350.041.4804438
TGCGGGT350.001254816739.9989935
ACCTCGG350.001254816739.99899344
AGCGCAC350.001254816739.9989937
ATCTCGG1051.4551915E-1136.66574542
GTCGCCG1151.8189894E-1236.5208251
CGGTGGT1350.036.29538346
Produced by FastQC (version 0.11.5)