FastQCFastQC Report
Wed 25 May 2016
SRR1781053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369068
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5013213.583404684231631No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG76592.0752273293810357No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC29180.7906402072246849No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC15920.4313568231328644No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT14240.38583675637009984No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT9770.26472086444774406No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT9460.2563213283189006No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC8930.24196083106636174No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG4860.13168305027799757No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4690.12707685304605112No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC4090.11081968634506378No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTG150.002224592369.9929530
AGGGGGG62550.064.3420570
AAACTCG850.053.5240236
CGTATGA1900.053.41567241
GTATGAC1450.053.09810342
TCCATAC200.00693247752.53033
TTCGGAG200.00693247752.53032
GCAACGT200.006947358652.50182713
CGTCTAC1000.052.4947248
ATCGTCC200.006951082552.49471729
AATCGTC200.006951082552.49471728
TATGACG1550.051.93025643
TATGCCG82250.051.4841843
GCCGTCT82350.051.4216646
TGCCGTC82150.051.4190545
ATGCCGT82550.051.38186644
TCGTATG85000.051.34189240
AACTCGT750.051.32816737
TCTCGTA83200.051.3169538
CCGTCTT81650.051.3053147
Produced by FastQC (version 0.11.5)