FastQCFastQC Report
Wed 25 May 2016
SRR1781052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254272
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6887327.086348477221243No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCGT150.002222931269.999848
GGCAACG200.006945919252.4998534
AACGCTC200.006945919252.4998532
CGAAGGA200.006945919252.4998537
TGCGAGC450.004302272631.11102757
GTCGCCG802.4586916E-630.62491451
CGGTGGT1052.0134394E-829.99991646
TCGGTGG1103.1650416E-828.63628445
CTGTTGT657.933214E-426.9282971
GTATAGA657.933214E-426.9282971
GTATAAG657.933214E-426.9282971
CGCCGTA657.942338E-426.92353
GGTGGTC1101.0117219E-625.45447547
TGGTCGC1101.0117219E-625.45447549
TCGCCGT700.001223948324.99993152
ATGAGAC700.001223948324.99993169
TCTTAGG901.9231664E-423.333272
GCCGTAT750.001829155523.33326754
TCTCGGT952.7732144E-422.10520243
AGAGCAA800.002661536221.87493918
Produced by FastQC (version 0.11.5)