FastQCFastQC Report
Wed 25 May 2016
SRR1781052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254272
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4364417.164296501384342No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG67592.658177070224012No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC23810.9363988170148503No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC13060.5136232066448527No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT9170.36063742763654666TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC7260.28552101686383086No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT6340.24933929020891013No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT4750.18680782783790587No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG4190.16478416813491065No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT3370.1325352378555248No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT3160.12427636546690159No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3080.1211301283664737No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC2860.11247797634029699No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2850.11208469670274351No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2850.11208469670274351No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2760.10854517996476214No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT2570.10107286685124592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG455.456968E-1270.00963610
GTTCGCT150.00222169370.00963611
TTCGTGA207.9190795E-569.9958727
AGGGGGG51300.064.6745570
TAAGACG352.9678995E-759.9964643
GCGTATG352.9678995E-759.9964640
TCACGTA1150.057.82267838
GCCGTAA553.6379788E-1157.2693546
CGTCTAC252.3881804E-455.99669648
TCGCGTA252.3881804E-455.99669638
ATCACGT1200.055.41339537
CGTCTTA4150.054.81604448
CGTCATA452.6937414E-854.4412348
CACGTAT1100.054.08771539
GCCGTCT66700.052.8379646
TGCCGTC69050.052.81368345
CCGTCTT66550.052.7992947
ATGCCGT70750.052.68240444
TATGCCG70900.052.66967443
ACCTTAC200.00693666252.5175556