FastQCFastQC Report
Wed 25 May 2016
SRR1781051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441027
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8218218.634233278234667No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT207.9235266E-569.9994343
GGACCTA252.3841609E-456.0249561
GCGCGTG252.3895114E-455.9995466
GGCGCGT252.3895114E-455.9995465
GTGAACA200.00694631852.5055272
TCGTTCA200.006949433652.49957341
TGTGCGC200.006949433652.4995733
ACGGCAA200.006949433652.49957316
CGTATCA800.048.12460756
TGGCGGC305.875676E-446.66628619
CTGCGGG305.875676E-446.66628618
TCCCGGC551.3102363E-744.5450935
TCGCCGT802.910383E-1143.7496452
GTCGCCG901.8189894E-1242.7774351
GCCGTAT757.548806E-1041.9996654
TGGTCGC1000.041.9996649
CCCGGCG602.595134E-740.83300436
TCGGTGG1200.040.83300445
AGATCTC953.6379788E-1240.5259940
GCGCGTC350.001254977339.9996766
Produced by FastQC (version 0.11.5)