FastQCFastQC Report
Wed 25 May 2016
SRR1781051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441027
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5384612.209229820396484No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG85201.9318545123087703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC30420.6897536885496807No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC16940.38410346758815217No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT14090.3194815736904997RNA PCR Primer, Index 19 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC10990.24919109260884256No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT10700.24261553147539722No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC4770.10815664347080792RNA PCR Primer, Index 19 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG4750.10770315649608754No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT4630.10498223464776534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAG207.924033E-569.9985350
GCACGCT150.002224178369.9985339
CGACACT207.924033E-569.9985323
CGTAATC207.924033E-569.9985348
CGACTTA150.002224178369.9985348
TAAGACG207.924033E-569.9985343
GCCGTAA301.02298145E-769.9985346
AGGGGGG67100.063.1134370
TGCCGTA501.4551915E-1162.9986745
AGCCGAC308.37261E-658.33210445
ATGACGA308.37261E-658.33210444
GACCTTC252.3869885E-456.011521
TCTGCCG252.3896652E-455.99881743
GTCTGCC252.3896652E-455.99881742
CGTCTGC252.3896652E-455.99881741
CAGCTGC252.3896652E-455.99881759
AACTCGA252.3896652E-455.99881737
TGACGTC1650.055.15035245
TATGACG1850.054.8637143
GACCACG1850.054.8637118