FastQCFastQC Report
Wed 25 May 2016
SRR1781050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241994
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7013528.98212352372373No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG2610.10785391373339835No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGA150.0022228869.99917610
TGTATAC150.0022228869.9991763
CGCGTCT308.360779E-658.3326457
GCAAGTC200.00694576352.49938241
TTATACG200.00694576352.4993822
CCGCGTT200.00694576352.49938226
TTGGGCG200.00694576352.4993822
GCCGTAT707.2759576E-1249.9994154
TCGCCGT751.4551915E-1146.66611552
CGTCTGG551.3069803E-744.544939
GGTATAC404.561992E-543.785681
GTCGCCG901.8189894E-1242.77727551
TGGTCGC1150.042.60819249
TGCGCGT350.001253878139.9995275
CTTACGT350.001253878139.99952734
ACTGGAC350.001253878139.9995273
GTGGTCG1450.038.62023548
CTCGGTG1100.038.1813744
TCGGTGG1300.037.69186445
CGTATCA1051.2732926E-1136.66623756
Produced by FastQC (version 0.11.5)