FastQCFastQC Report
Wed 25 May 2016
SRR1781050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241994
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4360918.02069472796846No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG74823.0918121936907528No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC34411.421936081059861No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC15420.6372058811375488No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT12750.5268725670884402Illumina PCR Primer Index 3 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT10310.42604362091622106No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC9370.3871996826367596No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4640.19174029108159707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4400.18182268981875585No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG4400.18182268981875585No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTAATGCATCTCGTATGCCGT3820.1578551534335562No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC3720.15372281957403902No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT3480.1438052183111978No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC3400.14049935122358406No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC3060.12644941610122565No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT2640.1090936138912535No Hit
CTTATACACATCTCCGAGACCACGAGACTTTAATGCATCTCGTATGCCGT2600.10744068034744664No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG2500.10330834648792946No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGACGT2460.10165541294412257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC150.002221292670.011787
AAACTCG150.002223114569.99731436
GCACGAC150.002223114569.99731442
CGTCTAC500.069.99731448
CGACTTC207.917496E-569.99731448
CGACTTA207.917496E-569.99731448
GCCGTAT207.917496E-569.99731446
TGCCGTA301.0211443E-769.99731445
AGGGGGG53200.064.01070470
AGCTTGA1050.059.99769655
GCCGACT352.9668809E-759.99769646
CCGACTT352.9668809E-759.99769647
AACTCGT700.059.99769637
CGTCTAA308.362096E-658.33109748
AATCTCG1300.056.53629336
AAGCCGT750.055.99785244
GCCGACA252.3877058E-455.99785246
TCTACGA750.055.99785211
AACTCGA252.3877058E-455.99785237
TGCCGAC509.767973E-1055.99785245
Produced by FastQC (version 0.11.5)