FastQCFastQC Report
Wed 25 May 2016
SRR1781049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195533
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6701134.2709414779091No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG2080.10637590585732332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG150.002222200669.999494
GACGCGT150.002222200669.999495
CGCGTCT301.0198892E-769.999497
ACGCGTC150.002222200669.999496
GCGCGGC401.0610165E-861.24955415
TGTGCGC252.3862014E-455.9995883
GCCGGCG452.6882844E-854.44404220
GAGCTAT200.006943654752.49961517
GCGTTTC200.006943654752.49961541
TGCGCGT200.006943654752.4996155
CGGTATA200.006943654752.4996152
GTGCGCG352.0811538E-549.9996344
TCTGGCG651.8371793E-1048.46118511
CCGGCGG551.3052158E-744.54512821
CGTATCA602.5852205E-740.83303556
GTATCAT602.5852205E-740.83303557
GGCGCGG953.6379788E-1240.5260214
GCGGCCG459.1743015E-538.88860317
GCCGTAT556.6710745E-638.18153854
GTCTGGC654.842859E-737.6920310
Produced by FastQC (version 0.11.5)