FastQCFastQC Report
Wed 25 May 2016
SRR1781049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195533
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4152421.236313052016794No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG69113.53444175663443No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC28261.4452803363115176No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC12720.6505295781274771No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT11030.5640991546184021RNA PCR Primer, Index 34 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT8660.44289199265597107No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC8160.41732086144026836No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4250.21735461533347314No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT3720.19024921624482824No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT3700.18922637099620013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3590.18360072212874554No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG3530.1805321863828612No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT3160.1616095492832412No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT3040.15547247779147255No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3020.15444963254284444No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC2930.14984682892401793RNA PCR Primer, Index 34 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACCTCATGGGATCTCGTATGCCGT2800.14319833480793523No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC2640.13501557281891036No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2380.12171858458674494No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT2250.11507009047066225RNA PCR Primer, Index 34 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT2240.11455866784634819No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT2240.11455866784634819No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGATCTCGTATGCCGTC2120.10842159635457954No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2110.10791017373026547No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCAGT2010.10279594748712492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC150.002223005869.99309545
CGAATTA150.002223005869.99309548
ATCGGAT252.8338363E-669.99309532
CGATCTC252.8338363E-669.99309535
CGACTTC401.3460522E-1069.9930948
TAATCCG207.916063E-569.9930943
AGGGGGG49900.063.8914970
GCCGACT501.4551915E-1162.9937946
TGCCGAC453.8380676E-1062.21608445
CACCGAG700.060.02478412
CGGATCT352.9651346E-759.9940834
TCGGATC308.359102E-658.32757633
CCGACTT553.6379788E-1157.2670847
ACCGAGC750.056.02313213
AAGCCGA252.387278E-455.99447644
GCGATCT252.387278E-455.99447634
GCCGTCA2000.054.2446546
CGTATGA1550.054.188241
TATGACG1750.053.9946743
CGTCATC2100.053.3280748
Produced by FastQC (version 0.11.5)