FastQCFastQC Report
Wed 25 May 2016
SRR1781048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438163
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6690115.268518793234481No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGT207.923727E-569.9989722
CATCGTT352.0855234E-549.99926432
TCGTTGT404.590527E-543.7493634
GCGTTTT350.001255009239.99941313
GAGCGCG350.001255009239.99941341
CAATACG459.193373E-538.88831769
TGCCGGC501.708907E-434.9994919
CGTGCGA400.002417979334.99948510
TCTTACG400.002417979334.99948518
CTAAGCG400.002417979334.9994859
AGCGCGG400.002417979334.99948542
TGTACCA400.002417979334.9994855
GTACAGC652.0881924E-532.336741
CCAAACG552.9907114E-431.8177179
CGTTACA450.004305889431.11065514
TGTTCGT450.004305889431.11065510
TAAGCGT450.004305889431.11065510
CTGATAC450.004305889431.1106553
GATCCTA604.953186E-429.1928921
TCGTTAC604.9797463E-429.1662413
Produced by FastQC (version 0.11.5)