FastQCFastQC Report
Wed 25 May 2016
SRR1781048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438163
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT408899.331915291797802No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG66891.5266008311975225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC27260.6221429011577884No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC14320.3268190148415087No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT10590.24169087759578056No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT10430.23803926849140616TruSeq Adapter, Index 10 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC7610.17367965802680738No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT5000.11411278451169998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTCG150.002224184369.99840523
ACGAATA150.002224184369.99840547
AGGGGGG52250.063.5679370
AAACTCG1050.063.3318936
AACTCGT950.062.63014637
ACAACGG1050.056.66537527
ACACCGT252.3896681E-455.99872627
ATGACGA252.3896681E-455.99872644
CCGACTT509.804353E-1055.99872647
ACCGGAT452.6990165E-854.443229
CGGATAA653.6379788E-1253.8449231
GTACTAG200.00694353752.5107881
ATCGTAT2000.052.49880239
GACATCG407.4748095E-752.49880222
AACTCGA200.00694980852.49880237
GAACACG407.4748095E-752.49880218
GCCGTCA1000.052.49880246
CGTATGC71550.051.26367641
TCGTATG72300.051.16757240
TATGCCG70000.051.09883543