FastQCFastQC Report
Wed 25 May 2016
SRR1781047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92947
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6708172.17123737183556No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG2410.2592875509699076No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG990.10651231346896618No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950.10220878565203825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATATC150.002218522469.9994668
CGCGGGC150.002218522469.99946616
CGCTGGC252.3796654E-455.99957316
TGCCGGC252.3796654E-455.99957319
GCGTGTG252.3796654E-455.9995738
CTGCCGG252.3796654E-455.99957318
TCTCGGT653.6379788E-1253.84573743
CTCGGTG653.6379788E-1253.84573744
CGGTGGT850.053.52946
GGCGTGT200.00693225152.4995967
GCGCGTC200.00693225152.4995966
GTCTGAC200.00693225152.49959610
CGGGCGC200.00693225152.49959612
GCGCTGC200.00693225152.49959615
GTCTCGG200.00693225152.49959642
CGCGTCT200.00693225152.4995967
CCGGCGG200.00693225152.49959621
TCGGTGG707.2759576E-1249.99961545
GCTGGCG352.0726451E-549.99961517
GTCTGGC352.0726451E-549.99961510
Produced by FastQC (version 0.11.5)