FastQCFastQC Report
Wed 25 May 2016
SRR1781047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92947
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4105744.17248539490247No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG69827.511807804447695No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC27632.9726618395429654No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC12551.350231852561137No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT9361.0070255091611349RNA PCR Primer, Index 14 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT9190.9887355159391912No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC8000.8607055633855853No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3830.412062788470849No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT3760.4045316147912251No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT3490.3754828020269616No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG3310.35611692685078594No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT3040.3270681140865224No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT3020.3249163501780585No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT3020.3249163501780585No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3010.3238404682238265No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT2980.32061282236113053No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT2790.3001710652307229No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC2710.291564009596867No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT2510.2700463705122274No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC2430.2614393148783715No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG2410.2592875509699076No Hit
CTTATACACATCTCCGAGCCAACGAGACCGGTTCCCATCTCGTATGCCGT2290.24637696751912383No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT2270.24422520361065983No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG2140.23023873820564406No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1770.19043110589906076No Hit
CTTATACACATCTCCGAGACCACGAGACCGGTTCCCATCTCGTATGCCGT1710.18397581417366887No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCATCTCGTATGCCGTC1590.1710652307228851No Hit
CTTATACACATCTCAGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1460.15707876531786932No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGA1140.12265054278244591No Hit
TCTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1080.11619525105705403No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGGTTCCCATCTCGTATGCCGT1080.11619525105705403No Hit
TATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG970.10436054956050222No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTAAGCCGT970.10436054956050222No Hit
CTTATACACATCTCCGAGCACACGAGACCGGTTCCCATCTCGTATGCCGT960.10328466760627024No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGAATGCCGT950.10220878565203825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGAG150.002214192270.033937
ATTTATA150.002214192270.033932
ACCGGAA150.002218928469.9962427
TATGCAT150.002218928469.9962453
CATTTCC150.002218928469.9962466
TCTATAT150.002218928469.996241
CTGAATG301.01432306E-769.9962454
AAACGCC150.002218928469.9962416
GCCGAAA150.002218928469.9962446
CCGAAAT150.002218928469.9962447
ACTCGAA207.892483E-569.9962338
TCCCAAC401.3278623E-1069.9962333
GCCCAAG353.6652636E-969.9962318
AACGAGC207.892483E-569.9962313
GATACCA207.892483E-569.9962331
TTCTGAA252.821378E-669.9962352
CGGATAC207.892483E-569.9962329
GGATACC207.892483E-569.9962330
ATCAGCT252.821378E-669.9962352
GGTTACA207.892483E-569.9962330
Produced by FastQC (version 0.11.5)