FastQCFastQC Report
Wed 25 May 2016
SRR1781046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192939
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6502533.70236188639933No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG1940.10054991473989187No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAG150.002220034870.0163358
CGAGCTA150.002222319469.99818428
ACGAGCT150.002222319469.99818427
GTCGGGC200.00693690852.51225310
TCGTTCG200.006944025452.49864223
TAGACGG200.006944025452.49864246
CGTTCGG200.006944025452.49864224
CATAGCT200.006944025452.49864270
TTCGGTA200.006944025452.49864226
GACGAGC200.006944025452.49864226
GCGCTGG352.0812784E-549.99870715
GGCGCTG703.8198777E-1044.99883714
TGCCGGC404.581231E-543.74886719
CACCGAC350.001251735440.0093357
CGAGTAC350.001253332739.9989661
GTCTCAG350.001253332739.99896629
GTCTAAA350.001253332739.9989661
CGGATCG350.001253332739.99896637
CCGAAAC350.001253332739.99896617
GCCGTAT459.1748545E-538.88788254
Produced by FastQC (version 0.11.5)