FastQCFastQC Report
Wed 25 May 2016
SRR1781046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192939
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT145767.554719367261155No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT42702.2131347213367953No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG15610.8090640046854187No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT10260.5317742913563355No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT8520.44159034720818496No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG7420.3845775089536071No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT7200.3731749413026915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC6790.3519247015896216No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT6460.33482085011324825No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG5730.296985057453392No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT5120.26536884714858067No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT4470.231679442725421No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT4430.22960624860707268No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT3640.18866066476969404No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC3430.17777639564836553No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT3430.17777639564836553No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCCT3180.16481893240868875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3170.16430063387910168No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCG2860.14823337946190246No Hit
CTTATACACATCTCCGAGCCCACGAGACATCGCGGCATCTCGTATGCCGT2710.1404589015180964No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCG2600.1347576176926386No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGG2560.13268442357429033No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT2440.12646484121924545No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGG2240.11609887062750404No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTAAGCCGT2130.11039758680204624No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCACGTATGCCGT2120.10987928827245917No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGA2000.10365970591741433No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCC1970.1021048103286531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA150.002223070569.99222648
CACGGAT150.002223070569.99222639
TAGACTT150.002223070569.99222624
ATAGCGT150.002223070569.99222644
AACGCGG150.002223070569.99222629
CTCGAAA150.002223070569.99222639
CGTAAAA207.916282E-569.99222648
CCGAATT301.0205622E-769.99222647
ACCGTCA150.002223070569.99222646
CAACTCG150.002223070569.99222636
CGCGTCA150.002223070569.99222631
GACGACA207.916282E-569.99222646
CCTATGC207.916282E-569.99222641
GAACACG207.916282E-569.99222618
TCGCGTC150.002223070569.99222630
TCGCTGC252.8339036E-669.9922230
AGGGGGG32550.065.26156670
GATGACG650.064.608243
GCCGGAA501.4551915E-1162.99346
TACGTAA501.4551915E-1162.99346
Produced by FastQC (version 0.11.5)