FastQCFastQC Report
Wed 25 May 2016
SRR1781045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132718
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6383248.09596286863877No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG2230.16802543739357134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT455.456968E-1270.0000152
GCCGTAT455.456968E-1270.0000154
CGTATCA401.3460522E-1070.056
TGGCGGC150.002220558770.019
TGCGCGG150.002220558770.05
CTCGGTG550.070.044
GGGCGCC150.002220558770.050
TCTCGGT500.070.043
GGTGCGC150.002220558770.03
GAACCAA150.002220558770.061
TTTGAGC150.002220558770.016
TTGCAGA150.002220558770.030
CGCGTCT150.002220558770.07
GTGCGCG150.002220558770.04
ATACCTT150.002220558770.013
TGGTCGC550.070.049
TCGGTGG700.065.045
CGGTGGT650.064.6153946
GTCGCCG550.063.6363651
TGGCGCT550.063.6363613
Produced by FastQC (version 0.11.5)