FastQCFastQC Report
Wed 25 May 2016
SRR1781045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132718
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3945729.72995373649392No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG64184.835817296824847No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC25241.9017766994680452No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT12740.9599300773067707No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC11590.8732801880679335No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT9750.7346403652857939No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT9500.7158034328425685Illumina PCR Primer Index 11 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC7450.5613405868081195No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3810.2870748504347564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3580.269744872586989No Hit
CTTATACACATCTCCGAGCCAACGAGACGACGCTCCATCTCGTATGCCGT3510.26447053150288585No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG3100.233577962295996No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT2770.20871321147093838No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2760.20795973417320934No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT2710.20419234768456426No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2630.1981645293027321No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2470.18610889253906782No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT2370.1785741195617776Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAGT2070.155969800629907No Hit
CTTATACACATCTCCGAGACCACGAGACGACGCTCCATCTCGTATGCCGT1870.14090025467532663No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG1790.1348724362934945No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG1660.12507723142301724No Hit
CTTATACACATCTCAGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1540.11603550385026898No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG1500.11302159465935292No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTACATCTCGTATGCCGT1420.10699377627752076No Hit
CTTATACACATCTCCGAGCACACGAGACGACGCTCCATCTCGTATGCCGT1390.1047333443843337No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG207.890408E-570.0222412
AAACTCG150.002221079569.9958636
GAAGCTC600.069.9958629
GCGCCAT150.002221079569.9958632
TGACGAC500.069.9958645
AGACGCA150.002221079569.9958625
CGCAGCT150.002221079569.9958628
TTTGCCT150.002221079569.9958646
CGATCCA252.8281538E-669.9958631
GCTCCTT150.002221079569.9958625
TGCCGAC401.3460522E-1069.9958645
AACACGT150.002221079569.9958637
GCTCAAT1250.067.1960232
GCTCCAA900.066.1071932
AGGGGGG49900.064.9460570
AGACGAA650.064.6115625
AATCTCG1300.064.6115636
GACGAAG650.064.6115626
ACGAAGC650.064.6115627
AACTCGT600.064.16286537
Produced by FastQC (version 0.11.5)