FastQCFastQC Report
Wed 25 May 2016
SRR1781044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312217
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6297420.169945903009765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCT150.002223523869.9988843
CGCTTGA150.002223523869.9988852
GCCGCTT200.006947759552.49915750
GTTGTCG200.006947759552.49915762
TTCGGCC200.006947759552.49915741
AGTCGAG200.006947759552.49915711
CGATTTC305.8732525E-446.6659242
CGTATCA851.8189894E-1245.29339256
GTCGCCG855.638867E-1141.1758151
GCCGTAT855.638867E-1141.1758154
GGTAGTG350.001248545240.0378341
GATAGTC350.001254463139.99936352
TCGCCGT901.03682396E-1038.88826452
GTATCAT1100.038.18120657
TCTCGGT1400.037.499443
TGGTCGC852.566594E-937.0582349
TACGGTG400.002416934434.9994428
CTCGGTG1003.237801E-1034.9994444
CGGATAC400.002416934434.9994438
GAAGTGT400.002416934434.999446
Produced by FastQC (version 0.11.5)