FastQCFastQC Report
Wed 25 May 2016
SRR1781044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312217
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3984712.762597808575446No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG67832.172527440850434No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC27670.8862425812816086No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC11070.35456109052357815No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT10340.33117991653241174No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT10050.3218915049468799TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC7300.233811739911664No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4470.14316965443906002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3860.12363196110397577No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT3850.12331167104930225No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT3350.10729716831562663No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG3130.10025078711280937No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGACG150.002216931770.051295
CGTATCA150.002223987669.995241
CACGGTA150.002223987669.995212
GTCTGCC150.002223987669.995242
CTGCCGT150.002223987669.995244
TCGTATC252.8373015E-669.995240
AGGGGGG50500.063.68869470
CGTCTTA650.059.22670448
AGACGCA452.6964699E-854.44070425
GCCGTAT452.6964699E-854.44070446
ATCGTAT653.6379788E-1253.8424639
ACGGAGA1700.053.52573827
GGCCCGA200.00694920452.496427
GCCGACA200.00694920452.496446
ACGGCCC200.00694920452.496422
ATACGAG200.00694920452.496412
TGCCGTA407.470171E-752.496445
CGTATGA1400.052.49639541
CGTCTAC750.051.3298148
AAGCCGT750.051.3298144
Produced by FastQC (version 0.11.5)