FastQCFastQC Report
Wed 25 May 2016
SRR1781043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124675
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6739954.05975536394626No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG2830.22699017445357933No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGC207.900961E-570.01
GCACGCT150.002220241470.016
TTGTACG150.002220241470.02
CCGCTTG150.002220241470.067
GGATAAG150.002220241470.025
TATGCGC150.002220241470.03
GCCGCTT150.002220241470.066
TGGCACC150.002220241470.04
CTTATGC207.900961E-570.01
TCTGTTC150.002220241470.03
TCTCGGG150.002220241470.043
CGGTGTA207.900961E-570.034
ATGCGCG150.002220241470.04
GCAGATC150.002220241470.08
TGTACGC150.002220241470.03
TGGCGCT501.4551915E-1162.99999613
CCGGCGG352.9543298E-760.021
TGCGCGT308.336527E-658.3333325
CGCGTCT308.336527E-658.3333327
GGCGCTG553.45608E-1157.2727314
Produced by FastQC (version 0.11.5)