FastQCFastQC Report
Wed 25 May 2016
SRR1781043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124675
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT3824330.674152797272907No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG64975.211148987367155No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC30152.4182875476238217No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT17561.4084620012031281No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC11120.8919189893723681No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT7490.6007619811509926TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC6430.5157409264086625No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT6340.5085221576097855No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5780.46360537397232804No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT4780.3833968317625827No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT4500.360938439943854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4420.3545217565670744No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCG4090.3280529376378584No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2780.22297974734309203No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG2710.21736514938840984No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2530.2029276117906557No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC2500.20052135552436332No Hit
TTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2350.18849007419290156No Hit
TATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCT2060.1652295969520754No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG1990.15961499899739323No Hit
CTTATACACATCTCCGAGCCAACGAGACCATCCTGTATCTCGTATGCCGT1890.1515941447764187No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGA1860.14918788851012632No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGACGT1580.12672949669139763No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATTCCGT1560.12512532584720273No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG1510.12111489873671547No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCCT1460.1171044716262282No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCAGT1370.1098857028273511No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCAGTATCTCGTATGCCGT1340.10747944656105875No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGAATCTCGTATGCCGT1300.10427110487266894No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCC1280.10266693402847403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA252.8267295E-669.9975919
CGAGTAT150.002220544369.9975939
GACGTAA150.002220544369.9975946
AAGCGGG150.002220544369.9975970
AGGCGGA150.002220544369.9975961
TGACGAA207.902309E-569.9975945
GACCCTC301.01705155E-769.9975926
ACGGGGT150.002220544369.9975912
TGGCAAT150.002220544369.9975940
CTTTGCG150.002220544369.9975919
AGACAAC252.8267295E-669.9975917
ATGACGA207.902309E-569.9975944
ACGTAAT150.002220544369.9975947
GACGAAA150.002220544369.9975946
ATCGAGT207.902309E-569.9975937
AGGGGGG43100.065.2877770
ATCCAGT850.061.76258530
CAGTATC850.061.76258533
CCAGTAT850.061.76258532
TCTGCTA800.061.2478953
Produced by FastQC (version 0.11.5)