FastQCFastQC Report
Wed 25 May 2016
SRR1781042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274337
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6858424.999908871205854No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTTG150.002223139969.9996343
TGGTCGC301.0214171E-769.9996349
TCGGTGG650.059.23046545
CTCGGTG452.6935595E-854.44416444
CGGTGGT653.6379788E-1253.84587546
GTCCTGA200.00693655552.5188751
TGGGTCG200.006946566552.4997312
TCGCCGT352.0833468E-549.99973752
CCGGCGA451.6811282E-646.66642821
CACGTCT305.871686E-446.6664247
CGCGTGT305.871686E-446.6664247
TGCCGGC703.8198777E-1044.99976319
GGCGCGT551.3077806E-744.5452235
CCCAATG404.585758E-543.74977543
CGCGTCT404.585758E-543.7497757
CGTATCA350.001254130639.9997956
TGGCCGG350.001254130639.9997952
AAATTCG350.001254130639.9997940
ACGTCTG350.001254130639.999798
GCGCGTG459.183871E-538.8886876