FastQCFastQC Report
Wed 25 May 2016
SRR1781042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274337
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4279715.600156012495582No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG71722.614302846499014No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC31921.1635324436732923No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC12390.4516343037942384No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT11560.42137954413732015RNA PCR Primer, Index 19 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT11530.42028599860755206No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC7130.25989932090822604RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT5220.19027692217965494No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4530.16512537499498792No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC4250.15491895005048537No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT3650.13304803945512272RNA PCR Primer, Index 19 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3630.13231900910194394No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3200.11664485650860074RNA PCR Primer, Index 19 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG3190.11628034133201136No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC2960.10789649227045568No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT2790.10169973426843626No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC150.00221724370.0465245
AGTGGAC150.002222060270.008218
CACGAGT150.002223667769.99544550
CACGTTA150.002223667769.99544530
CGACTTA207.920429E-569.99544548
CTCTCGA150.002223667769.99544537
CGAGTTA600.064.1624928
AGGGGGG52800.064.1624970
AGCCGTC352.9689E-759.99609445
CGACTTC650.059.2269148
TAACCGA308.366389E-658.3295411
CGAATGC850.057.6433141
GCCGACT800.056.871346
ACGCGAA509.786163E-1055.99635327
CCGACTT750.055.99635347
GCGATAA2150.055.34523429
AAGCCGT452.6948328E-854.44090344
GCCGTCA1100.054.08738746
GACCACG1300.053.84264418
CGCGATA2150.053.71743428