FastQCFastQC Report
Wed 25 May 2016
SRR1781041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198809
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7485337.65070997791851No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG2820.14184468509976914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCC150.002220544570.0130847
TACGCGT252.8292088E-670.0130845
ATACGCG150.002220544570.0130844
ACGCGTC301.0186159E-770.0130846
GTCGGGC207.915059E-569.99547610
CGCGTCT700.060.011217
CCGGCGA509.767973E-1055.99637621
TCGGGCG252.3869761E-455.99637611
CGTCTGA252.3869761E-455.9963769
TGCGCGT407.4431773E-752.509815
ACGTCCA200.00693849452.509818
AGCCGCC200.006945401452.49660562
CGTATCA608.367351E-1152.49660556
TCTCGGG200.006945401452.49660543
TCCGTTC200.006945401452.49660523
CGTCCGA200.006945401452.49660510
CGCCAAC200.006945401452.49660515
GCCGTAT750.051.33001354
TATACGC352.0757978E-550.0219273
TGCCGGC707.2759576E-1249.99676519
Produced by FastQC (version 0.11.5)