FastQCFastQC Report
Wed 25 May 2016
SRR1781041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198809
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4613523.20568988325478No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG77523.8992198542319514No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC31851.6020401490878178No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC15680.7886966887816951No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT13170.6624448591361558No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT10660.5361930294906166No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC9650.485390500430061No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT9600.4828755237438949No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5300.26658752873360864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4840.24344974322088034No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG4390.22081495304538526No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC4150.2087430649517879No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3670.18459928876459314No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAAATAAATCTCGTATGCCGT3630.18258730741566026No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC3610.18158131674119382No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAAAAAATCTCGTATGCCGT3120.15693454521676584No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT2990.15039560583273393No Hit
CTTATACACATCTCCGAGACCACGAGACCCAAATAAATCTCGTATGCCGT2950.14838362448380105No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC2650.13329376436680432No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAAATAAATCTCGTATGCCGT2590.13027579234340497No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCAGT2420.12172487161044017No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGACGT2350.1182039042498076No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG2340.11770090891257438No Hit
CTTATACACATCTCAGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2330.11719791357534116No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG2220.1116649648657757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATC150.002216210170.047576
AATGCGT150.002216210170.047576
AGCGTTA150.002220639570.0123210
TCGCCGA150.002222856769.9947236
AACGTAT150.002222856769.9947239
GCGTTAT150.002222856769.9947211
ATATCGA207.915481E-569.9947237
GGGCGGT150.002222856769.9947260
GTACGCG150.002222856769.9947269
ACGAATG150.002222856769.9947240
CGTCTAC850.065.8773848
AGGGGGG57450.064.69485570
TGACGAC550.063.63156545
CACCGAG1400.062.4952812
ACGTATG900.062.21753340
AAGCCGT800.061.2453844
AACTCGA401.0617441E-861.2453837
CACGTAT750.060.6620939
TGACGTC1450.060.3402845
AACACCG352.9597686E-760.01056310
Produced by FastQC (version 0.11.5)