FastQCFastQC Report
Wed 25 May 2016
SRR1781040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126805
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7028955.430779543393406No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG2500.19715310910453057No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA150.0022203370.01
GACGTTA150.0022203370.069
TCGGGCG150.0022203370.011
AAGCGCA150.0022203370.048
AGACGTT150.0022203370.068
AGCGCAA150.0022203370.049
CGTAAAG150.0022203370.063
ACGTAAA150.0022203370.062
GGCGCGT150.0022203370.05
TCCCCGG150.0022203370.013
GTAAGCG150.0022203370.046
CAGTAAA150.0022203370.04
TATACCG150.0022203370.06
TAAGCGC150.0022203370.047
ATACCGC150.0022203370.07
GTCTGGC308.337354E-658.33333210
CGGTGGT850.057.6470646
TCGGTGG850.057.6470645
ATATGCT252.3829055E-455.99999613
ATTCAAG252.3829055E-455.99999632
Produced by FastQC (version 0.11.5)