FastQCFastQC Report
Wed 25 May 2016
SRR1781040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126805
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4219233.27313591735342No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG71855.666180355664209No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC33052.606364102361894No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC13781.0867079373841726No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT9560.753913489215725TruSeq Adapter, Index 7 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT7670.6048657387326999No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC7150.5638578920389574No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT4930.3887859311541343No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4430.34935530933322817No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT4400.34698947202397384No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT4070.3209652616221758No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG3630.2862663144197784No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3550.2799574149284334No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3520.277591577619179No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT3280.25866487914514413No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT3060.24131540554394545No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2900.22869760656125548No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT2870.2263317692520011No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC2850.22475454437916484No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2690.2121367453964749No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT2590.2042506210322937No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT2030.16008832459287883No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT1860.14668191317377074No Hit
CTTATACACATCTCCGAGACCACGAGACCTTGGGTCATCTCGTATGCCGT1720.13564133906391704No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1660.1309096644454083No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA1490.11750325302630023No Hit
CTTATACACATCTCAGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1390.109617128662119No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1310.10330822917077402No Hit
TCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1290.10173100429793779No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1280.10094239186151964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA252.826433E-670.045
CAAACGA207.901489E-570.019
CAAGTAA150.0022203370.039
TGGAACC150.0022203370.04
GCAAACG207.901489E-570.018
CACGAAT150.0022203370.039
TGACGAA150.0022203370.045
CCGTCAA207.901489E-570.047
ATCGTAA150.0022203370.039
CCGTATA150.0022203370.047
CTAAACG150.0022203370.013
TGTTCAG150.0022203370.032
CAAATCG150.0022203370.036
GTATCTA150.0022203370.049
CGGTAGA150.0022203370.04
CGACTAC150.0022203370.048
GGACAAT150.0022203370.049
GCGTCAT207.901489E-570.032
GCTCCCA150.0022203370.01
ATTTCAC150.0022203370.029
Produced by FastQC (version 0.11.5)