FastQCFastQC Report
Wed 25 May 2016
SRR1781039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254019
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8742634.417110531102004No Hit
CTTATACACATCTGACGCTGCCGACGATTAGGCAAGTGTAGATCTCGGTG3010.11849507320318559No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATT150.002221416770.0118150
CACGTCT353.6998244E-969.998037
ACACGTC352.9672628E-759.9983146
ACGTCTG452.6930138E-854.4429138
CGCGTCT452.6930138E-854.4429137
CGTGACG200.00694660852.498522
GACCGTA200.00694660852.4985241
GTCTCGG200.00694660852.4985242
TGACCGT200.00694660852.4985240
CGTAGCA200.00694660852.4985256
ATGCACG200.00694660852.498524
TACACGT407.4625314E-752.498525
TGCACCG200.00694660852.498525
ACGCGTC200.00694660852.498526
GCGCGTG506.177288E-848.9986236
TGCGCGT651.8371793E-1048.460185
CGTATCA800.048.12364656
TGTGCGC751.4551915E-1146.6653563
GTGCGCG751.4551915E-1146.6653564
GCCGTAT1000.045.49872254
Produced by FastQC (version 0.11.5)