FastQCFastQC Report
Wed 25 May 2016
SRR1781039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254019
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5478521.567284336998412No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG93463.679252339391935No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC46301.8226983020955125No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT19120.752699601210933RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC18100.7125451245772954No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT13410.5279132663304714No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC10070.3964270389222853No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6370.2507686432904625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4980.1960483270936426No Hit
CTTATACACATCTCCGAGCCAACGAGACTTATGAAAATCTCGTATGCCGT4890.1925052850377334No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG4550.17912045949318753No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT4250.1673103193068235No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC3970.15628752179955044RNA PCR Primer, Index 19 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC3900.15353182242273217No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT3710.14605206697136827No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT3430.13502926946409521RNA PCR Primer, Index 19 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG3380.13306091276636786No Hit
CTTATACACATCTCCGAGACCACGAGACTTATGAAAATCTCGTATGCCGT3210.1263684999940949No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT3170.12479381463591306No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC3150.12400647195682213No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT3130.12321912927773118No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT3090.12164444391954933No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC2620.10314189096091238No Hit
CTTATACACATCTCAGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2600.10235454828182143No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA150.002223697769.993727
ATAACCG301.02170816E-769.993710
CCGAAAT150.002223697769.993747
GACGACA150.002223697769.993746
AGGGGGG68250.065.2761870
AACGGGG401.0635631E-861.24448469
TAACCGA352.968536E-759.994611
AAACACG352.968536E-759.994636
AACACGT352.968536E-759.994637
ATATCCG900.058.32808310
TATCCGA900.058.32808311
TCGAATG1150.057.8208840
CACGTAT1100.057.2675739
GGTCGCG252.388542E-455.99496541
TCGTATT252.388542E-455.99496540
CCGAGAC2450.055.70927414
ACGTATG1200.055.4116840
AAACTCG900.054.43954536
ACACGTA452.6942871E-854.43954538
TCCGAGA2650.054.14607213
Produced by FastQC (version 0.11.5)