FastQCFastQC Report
Wed 25 May 2016
SRR1781038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257335
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11064942.99803757747683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT252.8351005E-669.998457
TGCGCGT150.002223132469.998445
GCGCGTC252.3878008E-455.998766
TACGGAG252.3878008E-455.998765
GTACATG200.006914565352.5601161
TACATGC200.006935874552.5192452
GCGAACT200.006946547452.49883328
TGGACAC200.006946547452.49883339
TATATGC200.006946547452.49883363
GTGACAC200.006946547452.49883324
GGTACAA451.6809136E-646.6656330
TTCTGAA305.871494E-446.6656343
TCGGTGG305.871494E-446.6656345
GGCGCTG757.530616E-1041.9990714
CCATTGC503.4708573E-641.9990713
GACAGGC350.001254090339.9991116
TGGTCGG350.001254090339.9991125
GTCGGGT350.001254090339.9991127
TTGCGAT459.1832E-538.88802716
GCATTAG654.807207E-737.735471
Produced by FastQC (version 0.11.5)